Visisting Speaker in Chemistry ~ Professor Yi-Tao Long

Event Date: Wednesday, September 7, 2016

Professor Yi-Tao Long
School of Chemistry and Molecular Engineering
East China University of Science and Technology
Shanghai, 200237, P. R. China

will present a talk entitled:

Electrochemical Sensing of Single Nucleotide on Nanopore

Abstract:
Nanopore techniques have proven to be useful tools for single-molecule analysis.  Protein nanopores offer an inexpensive, label-free method for analysing single oligonucleotides.  The sensitivity of the approach is largely determined by the characteristics of the pore-forming protein employed and typically relies on nanopores that have been chemically modified or incorporate molecular motors.1   Over ten years study in our group, we show that a wild-type (WT) aerolysin nanopore can resolve individual short oligonucleotides that are 2 to 10 bases long.2-3  The sensing capabilities are attributed to the geometry of the aerolysin and the electrostatic interactions between the nanopore and the oligonucleotides.  Here, we reported that wild-type aerolysin nanopore exhibits a high current and temporary resolution for single oligonucleotide discrimination3.  Further applications of aerolysin nanopore were achieved to distinguish individual oligonucleotides from mixtures and to monitor the stepwise cleavage of oligonucleotides by exonuclease I.  Our findings reveal that aerolysin is a very promising candidate for the application of nucleic acid analysis at single-molecule level.

Reference:
1. Y.-L. Ying, J. Zhang, R. Gao and Y.-T. Long, Angew. Chem. Int. Ed., 2013, 52, 13154-13161.
2. C. Cao, Y.-L. Ying, Z.-L. Hu, D.-F. Liao, H. Tian and Y.-T. Long, Nat. Nanotechnol. 2016, DOI: 10.1038/nnano.2016.66.
3. C. Cao, J. Yu, Y.-Q. Wang, Y.-L. Ying and Y.-T. Long, Anal. Chem. 2016, 88, 5046-5049.

Visisting Speaker in Chemistry ~ Professor Yi-Tao Long